Our product Cell Counting Package deal-8 (CCK-8) current a further useful and delicate approach for the evaluation of cell amount dedication and cell proliferationtotoxicity assay than earlier strategies. The tools makes use of a extraordinarily water-soluble tetrazolium salt, WST-8, which produces a water-soluble formazan dye upon low cost inside the presence of an electron mediator. The amount of the formazan generated by dehydrogenases is straight in proportion to the numbers of residing cells. The detection sensitivity by CCK-Eight is bigger than completely different tetrazolium salts resembling MTT, XTT, MTS or WST-1.
Background
Cell proliferation shall be detected by numerous methods. Cell proliferation induces an increase inside the train of the mitochondrial dehydrogenases, which cleaves the tetrazolium salt to formazan.
The WST Cell Proliferation Colorimetric Assay Package deal plus provides the most effective and most delicate technique for quantification of cell proliferation and viability. Cell proliferation causes the rise inside the amount of formazan dye long-established that is straight proportional to the number of residing cells and shall be quantified by measuring the absorbance of the dye decision at 440 nm using microtiter plate reader. The tools might be utilized for the analysis of cell proliferation in response to pharmaceutical compounds, cytotoxic compounds like anticancer drugs and plenty of completely different toxic brokers.
The assay may additionally be used for the detection of cell proliferation in response to cytokines, mitogens, progress components and nutritional vitamins, and so forth. The assay might be very easy and straightforward, merely add-and-read, requiring no solubilization, no washing and no harvesting steps, and is regular, sooner and further delicate than MTS, MTT or XTT-based assays. Your total assay shall be carried out in a microtiter plate and correlates properly with the [3H]-thymidine incorporation assay.
| Choices | Fast and useful Simple one-step course of; takes decrease than 4 hours. Makes use of a water-soluble tetrazolium salt WST reagent. The technique is very easy that requires no washing/harvesting/or solubilization steps and is faster and further delicate than MTT/XTT/or MTS-based assays. Your total assay shall be carried out straight in a 96-well plate. |
| Supply | Gel pack |
| Storage Circumstances | Retailer at -20°C. |
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- 2. Xiangli Zhuang, Bo Wu, et al. “Influence of Xingbi Gel Nasal Drops on Fyn-STAT5 Pathway in Nasal Mucosa Fibroblasts of Guinea Pigs with Allergic Rhinitis.” Evid Based Complement Alternat Med. 2021 Mar 20;2021:6686815. PMID:33824677
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Cell Counting Kit-8 (CCK-8) |
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Cell Counting Kit-8 |
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Cell Counting Kit (CCK) |
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| 20-abx098245 |
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CCK-8 Cell Counting Kit |
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CCK-8 Cell Counting Kit |
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Cell Counting Kit-8 (CCK-8) |
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| AR1160 | 5 mL (for 500 assays, 10μL per well) | EUR 131 |
Cell Counting Kit-8 (CCK-8) |
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| B34302 | 5 mL (500 ractions) | EUR 130 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK-8) |
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| B34304 | 25 mL (2500 ractions) | EUR 315 |
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Description: The Bimake Cell Counting Kit-8 (CCK-8) is a fluorescent assay for the determination of cell viability in vitro. Results are obtained in only 3 steps: addition, incubation and reading. |
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Cell Counting Kit-8 (CCK8) Cell Viability Assay Kit |
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